{"id":16122,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/sensifast-sybr-no-rox-one-step-kit\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"sensifast-sybr-no-rox-one-step-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/sensifast-sybr-no-rox-one-step-kit\/","title":{"rendered":"SensiFAST\u2122 SYBR\u00ae No-ROX One-Step Kit"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-72001<\/td>\n100 x 20\u00b5l Reactions<\/td>\n<\/tr>\n
BIO-72005<\/td>\n500 x 20\u00b5l Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
Product Highlights<\/span><\/div>\n
\n
    \n
  • Sensitive \u2013 optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets<\/li>\n
  • Reproducible \u2013 consistent results between technical replicates for increased confidence in results<\/li>\n
  • Specific \u2013 antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability<\/li>\n
  • Robust \u2013 reliable detection of RNA targets from a broad range of sample types<\/li>\n
  • Fast \u2013 delivers reproducible, accurate assay results in as little as 40 minutes<\/li>\n<\/ul>\n<\/div>\n
    \n

    The SensiFAST\u2122 SYBR\u00ae<\/sup> No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.<\/p>\n

    An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.<\/p>\n

    The SensiFAST SYBR\u00ae<\/sup> No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.<\/p>\n

    Applications<\/h5>\n
      \n
    • Gene expression analysis<\/li>\n
    • Pathogen detection<\/li>\n
    • RNA viral pathogen detection<\/li>\n
    • Genetic profiling<\/li>\n
    • miRNA profiling \/ quantification<\/li>\n
    • Genetically modified organisms (GMO) characterization<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.<\/p>\n","protected":false},"featured_media":16334,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[287],"product_tag":[],"class_list":{"0":"post-16122","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-sybr-one-step-kits","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nSensiFAST\u2122 SYBR\u00ae No-ROX One-Step Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-sybr-no-rox-one-step-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SensiFAST\u2122 SYBR\u00ae No-ROX One-Step Kit - 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