{"id":16117,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/simplifi-hs-mix\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"simplifi-hs-mix","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/simplifi-hs-mix\/","title":{"rendered":"SimpliFi HS Mix"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-25060<\/td>\n100 x 50\u00b5l Reactions<\/td>\n<\/tr>\n
BIO-25061<\/td>\n500 x 50\u00b5l Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
\n
Product Highlights<\/h5>\n
    \n
  • Robust \u2013 optimized enzyme\/buffer mix promotes reliable amplification of a broad range of targets, including complex DNA extracted from human, animal and plant samples<\/li>\n
  • Specific \u2013 the aptamer-based hot-start remains completely inactive during PCR set-up and after amplification, to prevent non-specific products<\/li>\n
  • Optimized \u2013 high yields with minimal optimization, regardless of a template\u2019s GC content, reducing time to results and eliminating the cost of unnecessary repeats<\/li>\n
  • Enhanced accuracy \u2013 higher than 130x Taq fidelity, reducing errors for next generation sequencing (NGS) library amplification<\/li>\n<\/ul>\n<\/div>\n
    \n

    SimpliFi HS Mix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an aptamer-mediated hot-start polymerase, dNTPs and MgCl2<\/sub>. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors. The advanced buffer chemistry and enhancers has been developed for fast PCR and is designed for superior sensitivity and specificity, making SimpliFi HS Mix perfect for NGS library amplification.<\/p>\n

    Applications<\/h5>\n
      \n
    • Gene expression<\/li>\n
    • Viral and bacterial detection<\/li>\n
    • Robust PCR<\/li>\n
    • High-specificity PCR<\/li>\n
    • High-fidelity PCR<\/li>\n
    • GC\/AT-rich PCR<\/li>\n
    • Blunt end cloning<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
      A high-fidelity polymerase mix using aptamer-based hot-start technology, highly suited to amplification of DNA from crude samples, for fast, inexpensive target enrichment, NGS library amplification and cloning applications.<\/div>\n
      \n
      <\/h5>\n<\/div>\n","protected":false},"featured_media":16223,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[275],"product_tag":[],"class_list":{"0":"post-16117","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-fast-pcr","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nSimpliFi HS Mix - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/simplifi-hs-mix\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SimpliFi HS Mix - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"A high-fidelity polymerase mix using aptamer-based hot-start technology, highly suited to amplification of DNA from crude samples, for fast, inexpensive target enrichment, NGS library amplification and cloning applications.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/simplifi-hs-mix\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/SimpliFi_HS_Mix_Fidelity_fig11.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1035\" \/>\n\t<meta property=\"og:image:height\" content=\"655\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/simplifi-hs-mix\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/simplifi-hs-mix\/\",\"name\":\"SimpliFi HS Mix - 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