{"id":14879,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/nest-cell-culture-inserts\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"nest-cell-culture-inserts","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/nest-cell-culture-inserts\/","title":{"rendered":"Nest Cell Culture Inserts"},"content":{"rendered":"<div class=\"module_cont\">\n<div class=\"bossdesc bossdescbox clearfix\">\n<p><span style=\"font-size: 14px\">NEST <span class=\"keywords_replace\"><a style=\"color: unset;font-size: unset;font-family: unset;font-weight: unset;text-decoration: underline\">Cell Culture Inserts<\/a><\/span><\/span><span style=\"font-size: 14px\"> With ISO9001&amp;13485 certified, <\/span><span style=\"font-size: 14px\"><span style=\"font-size: 14px\">NEST Cell Culture Inserts<\/span><\/span><span style=\"font-size: 14px\"> are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials.\u00a0<\/span><span style=\"font-size: 14px\">Nest is committed to be your best choice for laboratory and medical consumables.<\/span><\/p>\n<p><span style=\"font-size: 14px\">Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof.\u00a0<\/span><span style=\"font-size: 14px\">In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, <span class=\"keywords_replace\"><a style=\"color: unset;font-size: unset;font-family: unset;font-weight: unset;text-decoration: underline\">Molecular Biology<\/a><\/span>, <span class=\"keywords_replace\"><a style=\"color: unset;font-size: unset;font-family: unset;font-weight: unset;text-decoration: underline\">Microbiology<\/a><\/span>, liquid handling and storage, <span class=\"keywords_replace\"><a style=\"color: unset;font-size: unset;font-family: unset;font-weight: unset;text-decoration: underline\">Lab Instruments<\/a><\/span> and <span class=\"keywords_replace\"><a style=\"color: unset;font-size: unset;font-family: unset;font-weight: unset;text-decoration: underline\">Medical Instruments<\/a><\/span>.<\/span><\/p>\n<p><span style=\"font-size: 14px\"><strong>Application\uff1a<\/strong><\/span><\/p>\n<div class=\"J_module\">\n<div class=\"detail-decorate-json-renderer-container\">\n<div class=\"magic-1\">\n<div class=\"magic-3\">\n<p><span style=\"font-size: 14px\">Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and<\/span><span style=\"font-size: 14px\">independent cell lines. <\/span><br>\n<span style=\"font-size: 14px\">This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out<\/span><span style=\"font-size: 14px\">metabolic activities in a more natural manner because the cells feed both apically and basolaterally.<\/span><\/p>\n<p><span style=\"font-size: 14px\"><span style=\"font-size: 14px\">Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.<\/span><\/span><\/p>\n<div class=\"text-title\"><span class=\"magic-33\" style=\"font-size: 14px\"><strong>Product Features\uff1a<\/strong><\/span><\/div>\n<p>\u00b7Innovative hanging design facilitates pipetting.<br>\n\u00b7Supplied in multiple well plates: 6-well, 12-well, 24well.<br>\n\u00b7PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds<br>\n\u00b7The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.<br>\n\u00b7Compatible with most solvents used for fixing and dyeing.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"87\"><strong>Packing<\/strong>\n<p><strong>specification<\/strong><\/p><\/td>\n<td width=\"113\"><strong>Inserts\/plate<\/strong><\/td>\n<td width=\"100\"><strong>Insert<\/strong>\n<p><strong>\u00a0Diameter(mm)<\/strong><\/p><\/td>\n<td width=\"85\"><strong>Well Volume(ml)<\/strong><\/td>\n<td width=\"95\"><strong>Insert Volume(ml)<\/strong><\/td>\n<td width=\"115\"><strong>Insert Membrane Growth Area<\/strong><strong>\uff08cm\u00b2<\/strong><strong>\uff09<\/strong><\/td>\n<\/tr>\n<tr>\n<td>6<\/td>\n<td>6 inserts\/6well<\/td>\n<td>24<\/td>\n<td>2.6<\/td>\n<td>1.5<\/td>\n<td>4.67<\/td>\n<\/tr>\n<tr>\n<td>12<\/td>\n<td>12\u00a0inserts\/6well<\/td>\n<td>12<\/td>\n<td>1.5<\/td>\n<td>0.5<\/td>\n<td>1.12<\/td>\n<\/tr>\n<tr>\n<td>24<\/td>\n<td>12\u00a0inserts\/6well<\/td>\n<td>6.5<\/td>\n<td>0.6<\/td>\n<td>0.1<\/td>\n<td>0.33<\/td>\n<\/tr>\n<tr>\n<td>100mm dish<\/td>\n<td>1\u00a0inserts\/6well<\/td>\n<td>75<\/td>\n<td>13<\/td>\n<td>9<\/td>\n<td>44<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u00a0<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"87\"><strong>Pore Size(um)<\/strong><\/td>\n<td width=\"113\"><strong>Membrane Density<\/strong><strong>\uff08well\/cm\u00b2<\/strong><strong>\uff09<\/strong><\/td>\n<\/tr>\n<tr>\n<td>0.4<\/td>\n<td>1\u00d710^8<\/td>\n<\/tr>\n<tr>\n<td>3.0<\/td>\n<td>2\u00d710^6<\/td>\n<\/tr>\n<tr>\n<td>5.0<\/td>\n<td>4\u00d710^5<\/td>\n<\/tr>\n<tr>\n<td>8.0<\/td>\n<td>1\u00d710^5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Cell Culture Insert, PC Membrane<\/strong><\/p>\n<p><strong>Individually packaged in peelable Tyvek film and Blister, sterile.<\/strong><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"87\"><strong>TC Treated Cat.NO.<\/strong><\/td>\n<td width=\"113\"><strong>Non-Treated\u00a0 Cat.NO.<\/strong><\/td>\n<td><strong>Description<\/strong><\/td>\n<td><strong>Pore Size<\/strong>\n<p><strong>(\u03bcm)<\/strong><\/p><\/td>\n<td><strong>Membrane<\/strong>\n<p><strong>Transparence<\/strong><\/p><\/td>\n<td><strong>Insert\u00a0 Packing<\/strong><\/td>\n<td><strong>Plate Packing<\/strong><\/td>\n<\/tr>\n<tr>\n<td>723001<\/td>\n<td>723011<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724001<\/td>\n<td>724011<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725001<\/td>\n<td>725011<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>723101<\/td>\n<td>723111<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724101<\/td>\n<td>724111<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725101<\/td>\n<td>725111<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>724201<\/td>\n<td>724211<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>5.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725201<\/td>\n<td>725211<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>5.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>723301<\/td>\n<td>723311<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Translucent<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724301<\/td>\n<td>724311<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725301<\/td>\n<td>725311<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>726001<\/td>\n<td>\/<\/td>\n<td>100 mm Cell Culture Insert-Dish<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>1<\/td>\n<td>10<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Cell Culture Insert, PET Membrane<\/strong><\/p>\n<p><strong>Individually packaged in peelable Tyvek film and Blister, sterile.<\/strong><\/p>\n<\/div>\n<\/div>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"87\"><strong>TC Treated<\/strong>\n<p><strong>Cat.NO.<\/strong><\/p><\/td>\n<td width=\"113\"><strong>Non-Treated<\/strong>\n<p><strong>Cat.NO.<\/strong><\/p><\/td>\n<td><strong>Description<\/strong><\/td>\n<td><strong>Pore Size<\/strong>\n<p><strong>(\u03bcm)<\/strong><\/p><\/td>\n<td><strong>Membrane<\/strong>\n<p><strong>Transparence<\/strong><\/p><\/td>\n<td><strong>Insert Packing<\/strong><\/td>\n<td><strong>Plate Packing<\/strong><\/td>\n<\/tr>\n<tr>\n<td>723121<\/td>\n<td>723131<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724121<\/td>\n<td>724131<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725121<\/td>\n<td>725131<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>0.4<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>723021<\/td>\n<td>723031<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724021<\/td>\n<td>724031<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725021<\/td>\n<td>725031<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>3.0<\/td>\n<td>Translucent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div>\n<table>\n<tbody>\n<tr>\n<td width=\"87\"><strong>TC Treated<\/strong>\n<p><strong>Cat.NO.<\/strong><\/p><\/td>\n<td width=\"113\"><strong>Non-Treated<\/strong>\n<p><strong>Cat.NO.<\/strong><\/p><\/td>\n<td><strong>Description<\/strong><\/td>\n<td><strong>Pore Size<\/strong>\n<p><strong>(\u03bcm)<\/strong><\/p><\/td>\n<td><strong>Membrane<\/strong>\n<p><strong>Transparence<\/strong><\/p><\/td>\n<td><strong>Insert Packing<\/strong><\/td>\n<td><strong>Plate Packing<\/strong><\/td>\n<\/tr>\n<tr>\n<td>723421<\/td>\n<td>723431<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>1.0<\/td>\n<td>Opaque<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724421<\/td>\n<td>724431<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>1.0<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>725421<\/td>\n<td>725431<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>1.0<\/td>\n<td>Opaque<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>723321<\/td>\n<td>723331<\/td>\n<td>6 Cell Culture Inserts + 6 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Transparent<\/td>\n<td>6<\/td>\n<td>24<\/td>\n<\/tr>\n<tr>\n<td>724321<\/td>\n<td>724331<\/td>\n<td>12 Cell Culture Inserts + 12 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Transparent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<tr>\n<td>724321<\/td>\n<td>724331<\/td>\n<td>12 Cell Culture Inserts + 24 Well Plate<\/td>\n<td>8.0<\/td>\n<td>Transparent<\/td>\n<td>12<\/td>\n<td>48<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<div class=\"module_cont\">\n<div class=\"bossdesc bossdescbox clearfix\">\n<p><strong>New addition:\u00a01\u03bcm &amp; 8\u03bcm\u00a0PET Membrane<\/strong><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" title=\"Cell Culture Inserts\" src=\"\/\/bsg-i.nbxc.com\/uploads\/32\/92\/da\/b65bd2ab99d0913f5cdf1b3f26.jpg\" alt=\"Cell Culture Inserts\" width=\"900\" height=\"948\"><\/p>\n<div class=\"J_module\">\n<div class=\"icbu-pc-images magic-28\">\n<p><img decoding=\"async\" title=\"Cell Culture Inserts\" src=\"\/\/bsg-i.nbxc.com\/uploads\/a5\/62\/cf\/024ddf3607e95ef73f96f552c2.jpg\" alt=\"Cell Culture Inserts\" width=\"900\" height=\"480\"><\/p>\n<\/div>\n<\/div>\n<div class=\"J_module\">\n<div class=\"icbu-pc-images magic-28\">\n<div>\n<div class=\"flex-layout-v\">\n<div class=\"flex-layout-h magic-28\">\n<div class=\"magic-34\"><img decoding=\"async\" title=\"Cell Culture Inserts\" src=\"\/\/bsg-i.nbxc.com\/product\/77\/b2\/7e\/c035d576cb99a8664584a3658f.jpg\" alt=\"Cell Culture Inserts\" width=\"900\" height=\"509\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"J_module\">\n<div class=\"icbu-pc-images magic-28\">\n<div>\n<div class=\"flex-layout-v\">\n<div class=\"flex-layout-h magic-28\">\n<div class=\"magic-34\"><img loading=\"lazy\" decoding=\"async\" title=\"Cell Culture Inserts\" src=\"\/\/bsg-i.nbxc.com\/uploads\/c4\/d5\/fa\/719199df5f0b12bb1eec8f4312.jpg\" alt=\"Cell Culture Inserts\" width=\"900\" height=\"480\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\"><strong>Attention\uff1a<\/strong><\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">Guidelines for us\uff1a<\/span><\/div>\n<p style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.\u00a0<\/span><\/p>\n<p style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.\u2022 Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.<\/span><\/p>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Check the volume of the culture medium regularly and add fresh medium as needed.<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.<\/span><\/div>\n<div style=\"font-family: arial\"><span style=\"font-size: 14px\">\u2022 Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.<\/span><\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>NEST Cell Culture Inserts With ISO9001&amp;13485 certified, NEST Cell Culture Inserts are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials.\u00a0Nest is committed to be your best choice for laboratory and medical consumables. Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof.\u00a0In [\u2026]<\/p>\n","protected":false},"featured_media":14878,"template":"","meta":{"inline_featured_image":false},"product_brand":[222],"product_cat":[233],"product_tag":[],"class_list":["post-14879","product","type-product","status-publish","has-post-thumbnail","product_brand-nest","product_cat-cell-culture-inserts","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Nest Cell Culture Inserts - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/nest-cell-culture-inserts\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nest Cell Culture Inserts - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"NEST Cell Culture Inserts With ISO9001&amp;13485 certified, NEST Cell Culture Inserts are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials.\u00a0Nest is committed to be your best choice for laboratory and medical consumables. 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