{"id":12439,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12439"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"murashige-skoog-medium-incl-mes-buffer","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/","title":{"rendered":"Murashige & Skoog medium incl. MES buffer"},"content":{"rendered":"
\n
\n
\n
\n

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White\u2019s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n
\n
\n

Composition<\/a><\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n
Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CoCl2<\/sub>.6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.025<\/td>\n0.10<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n6.20<\/td>\n100.27<\/td>\n<\/tr>\n
KI<\/td>\n0.83<\/td>\n5.00<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n16.90<\/td>\n100.00<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.25<\/td>\n1.03<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n8.60<\/td>\n29.91<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
CaCl2<\/sub><\/td>\n332.02<\/td>\n2.99<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n170.00<\/td>\n1.25<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n1900.00<\/td>\n18.79<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n180.54<\/td>\n1.50<\/td>\n<\/tr>\n
NH4<\/sub>NO3<\/sub><\/td>\n1650.00<\/td>\n20.61<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n
Buffer<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
MES<\/td>\n500.00<\/td>\n2.35<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Total concentration Micro and Macro elements including MES buffer: 4802.09 mg\/l<\/p>\n

Total concentration Micro and Macro elements including vitamins and MES: 4905.19 mg\/l<\/p>\n

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).<\/p>\n

Hazard statements<\/h4>\n
    \n
  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
      \n
    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White\u2019s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[202],"product_tag":[],"class_list":{"0":"post-12439","1":"product","2":"type-product","3":"status-publish","5":"product_brand-duchefa","6":"product_cat-ms-type-media","8":"first","9":"instock","10":"shipping-taxable","11":"product-type-simple"},"yoast_head":"\nMurashige & Skoog medium incl. MES buffer - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Murashige & Skoog medium incl. MES buffer - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White\u2019s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana [\u2026]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/\",\"name\":\"Murashige & Skoog medium incl. MES buffer - Filara Biomed\",\"isPartOf\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#website\"},\"datePublished\":\"2025-08-08T11:00:00+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/filarabiomed.ro\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Shop\",\"item\":\"https:\/\/filarabiomed.ro\/shop\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Murashige & Skoog medium incl. MES buffer\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#website\",\"url\":\"https:\/\/filarabiomed.ro\/en\/\",\"name\":\"Filara Biomed\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/filarabiomed.ro\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#organization\",\"name\":\"Filara Biomed\",\"url\":\"https:\/\/filarabiomed.ro\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png\",\"contentUrl\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png\",\"width\":491,\"height\":170,\"caption\":\"Filara Biomed\"},\"image\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Murashige & Skoog medium incl. MES buffer - Filara Biomed","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/","og_locale":"en_US","og_type":"article","og_title":"Murashige & Skoog medium incl. MES buffer - Filara Biomed","og_description":"MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White\u2019s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana [\u2026]","og_url":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/","og_site_name":"Filara Biomed","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/","url":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/","name":"Murashige & Skoog medium incl. MES buffer - Filara Biomed","isPartOf":{"@id":"https:\/\/filarabiomed.ro\/en\/#website"},"datePublished":"2025-08-08T11:00:00+00:00","breadcrumb":{"@id":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/filarabiomed.ro\/en\/product\/murashige-skoog-medium-incl-mes-buffer\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/filarabiomed.ro\/en\/"},{"@type":"ListItem","position":2,"name":"Shop","item":"https:\/\/filarabiomed.ro\/shop\/"},{"@type":"ListItem","position":3,"name":"Murashige & Skoog medium incl. MES buffer"}]},{"@type":"WebSite","@id":"https:\/\/filarabiomed.ro\/en\/#website","url":"https:\/\/filarabiomed.ro\/en\/","name":"Filara Biomed","description":"","publisher":{"@id":"https:\/\/filarabiomed.ro\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/filarabiomed.ro\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/filarabiomed.ro\/en\/#organization","name":"Filara Biomed","url":"https:\/\/filarabiomed.ro\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/","url":"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png","contentUrl":"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png","width":491,"height":170,"caption":"Filara Biomed"},"image":{"@id":"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/product\/12439","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:attachment":[{"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/media?parent=12439"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/product_brand?post=12439"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/product_cat?post=12439"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/filarabiomed.ro\/en\/wp-json\/wp\/v2\/product_tag?post=12439"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}