{"id":12432,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12432"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"westvaco-wv5-medium-incl-vitamins","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/westvaco-wv5-medium-incl-vitamins\/","title":{"rendered":"Westvaco WV5 medium incl. vitamins"},"content":{"rendered":"
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A significant improvement in the initiation of embryogenic cultures of loblolly pine from immature seeds was achieved on Westvaco\u2019s WV5 medium defined by Coke with the addition of 30 g\/l sucrose, 3 mg\/l 2,4-D, 0.5 mg\/l BA, 500 mg\/l casein hydrolysate, and 1.25 mg\/l Gelrite\u2122. Up to a threefold increase in embryogenic culture initiation was seen with WV5 medium over other published media. WV5 medium was also found suitable for embryo development. Shoot cultures of loblolly pine have also been established and micropropagated using Westvaco\u2019s WV5 medium. Seedling shoots cultured on WV5 medium with 20 g\/l sucrose, 5 g\/l activated charcoal, and 8 g\/l agar showed improved survival and shoot growth compared to that seen with other published media. Shoot quality was excellent and rooting response was good.<\/p>\n

Composition<\/a><\/h3>\n
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Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CoCl2<\/sub>\u00a0. 6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>\u00a0. 5H2<\/sub>O<\/td>\n0.25<\/td>\n1.00<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.71<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n31.00<\/td>\n501.37<\/td>\n<\/tr>\n
KI<\/td>\n0.83<\/td>\n5.00<\/td>\n<\/tr>\n
MnSO4<\/sub>\u00a0. H2<\/sub>O<\/td>\n15.16<\/td>\n89.69<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>\u00a0. 2H2<\/sub>O<\/td>\n0.25<\/td>\n1.03<\/td>\n<\/tr>\n
ZnSO4<\/sub>\u00a0. 7H2<\/sub>O<\/td>\n8.60<\/td>\n29.91<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
CaCl2<\/sub><\/td>\n452.88<\/td>\n4.08<\/td>\n<\/tr>\n
KCl<\/td>\n718.67<\/td>\n9.64<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n270.00<\/td>\n1.98<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n1084.06<\/td>\n10.72<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n903.79<\/td>\n7.51<\/td>\n<\/tr>\n
NH4<\/sub>NO3<\/sub><\/td>\n700.00<\/td>\n8.74<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n
Vitamins<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
myo-Inositol<\/td>\n1000.00<\/td>\n5549.39<\/td>\n<\/tr>\n
Thiamine HCl<\/td>\n0.40<\/td>\n1.19<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Total concentration Micro and Macro elements including vitamins: 5222.63 mg\/l<\/p>\n

Coke J.E, Basal nutrient medium for in vitro cultures of loblolly pine., United States Patent#5,534,433. July 9, 1996.<\/p>\n

Hazard statements<\/h4>\n
    \n
  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
      \n
    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
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      <\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n
      \n
      <\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      A significant improvement in the initiation of embryogenic cultures of loblolly pine from immature seeds was achieved on Westvaco\u2019s WV5 medium defined by Coke with the addition of 30 g\/l sucrose, 3 mg\/l 2,4-D, 0.5 mg\/l BA, 500 mg\/l casein hydrolysate, and 1.25 mg\/l Gelrite\u2122. Up to a threefold increase in embryogenic culture initiation was [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[201],"product_tag":[],"class_list":["post-12432","product","type-product","status-publish","product_brand-duchefa","product_cat-ptc-media","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nWestvaco WV5 medium incl. vitamins - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/westvaco-wv5-medium-incl-vitamins\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Westvaco WV5 medium incl. vitamins - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"A significant improvement in the initiation of embryogenic cultures of loblolly pine from immature seeds was achieved on Westvaco\u2019s WV5 medium defined by Coke with the addition of 30 g\/l sucrose, 3 mg\/l 2,4-D, 0.5 mg\/l BA, 500 mg\/l casein hydrolysate, and 1.25 mg\/l Gelrite\u2122. 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