{"id":12428,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12428"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"rugini-olive-medium","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/rugini-olive-medium\/","title":{"rendered":"Rugini Olive Medium"},"content":{"rendered":"
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The Olive (Olea europaea sativa L.) plays an important role in the economies of countries in the Mediterranean area. The in vitro culture of Olive, a particular difficult species to propagate in vitro, required the development of a specific medium formulation. Rugini medium is dedicated to the proliferation of the olive shoots. The medium has an enriched composition compared to MS. Olive tissues are characterized by a high content of Ca, Mg, S, Cu and Zn. The best nitrogen source is a combination of NO3- and NH4+ supplemented with glutamine 2,19 mg\/l. The better carbon source is mannitol (30-36 gr\/l) compared to sucrose. A better cytokinin to be used is zeatin: 1 mg\/l if filter sterilized, 3-4 mg\/l when autoclaved. TDZ and 2iP are less effective. Shoots grow more rapidly compared to other media. The proliferation rate increases and more tender, sturdier shoots with less basal callus are obtained.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Composition<\/a><\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n
Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CoCl2<\/sub>.6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.25<\/td>\n1.00<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n12.40<\/td>\n200.55<\/td>\n<\/tr>\n
KI<\/td>\n0.83<\/td>\n5.00<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n16.90<\/td>\n100.00<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.25<\/td>\n1.03<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n14.30<\/td>\n49.75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
CaCl2<\/sub><\/td>\n332.16<\/td>\n2.99<\/td>\n<\/tr>\n
Ca(NO3<\/sub>)2<\/td>\n416.92<\/td>\n2.54<\/td>\n<\/tr>\n
KCl<\/td>\n500.00<\/td>\n6.71<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n340.00<\/td>\n2.50<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n1100.00<\/td>\n10.88<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n732.60<\/td>\n6.09<\/td>\n<\/tr>\n
NH4<\/sub>NO3<\/sub><\/td>\n412.00<\/td>\n5.15<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n
Vitamins<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
Biotin<\/td>\n0.05<\/td>\n0.20<\/td>\n<\/tr>\n
Folic acid<\/td>\n0.50<\/td>\n1.13<\/td>\n<\/tr>\n
Glycine<\/td>\n2.00<\/td>\n26.64<\/td>\n<\/tr>\n
myo-Inositol<\/td>\n100.00<\/td>\n554.94<\/td>\n<\/tr>\n
Nicotinic acid<\/td>\n5.00<\/td>\n40.62<\/td>\n<\/tr>\n
Pyridoxine HCl<\/td>\n0.50<\/td>\n2.43<\/td>\n<\/tr>\n
Thiamine HCl<\/td>\n0.50<\/td>\n1.48<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Total concentration Micro and Macro elements including vitamins: 4023.89 mg\/l<\/p>\n

Rugini E., In vitro propagation of some olive cultivars, Scientia Horticulturae 24, 123 (1984)., Jacoboni A., Luppino M., Rugini E., Role of basal shoot darkening Scientia Horticolturae, 53:63 (1993)<\/p>\n

Hazard statements<\/h4>\n
    \n
  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n
  • H412<\/span>\u00a0Harmful to aquatic life with long lasting effects<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
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    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P273<\/span>\u00a0Avoid release to the environment<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      The Olive (Olea europaea sativa L.) plays an important role in the economies of countries in the Mediterranean area. The in vitro culture of Olive, a particular difficult species to propagate in vitro, required the development of a specific medium formulation. Rugini medium is dedicated to the proliferation of the olive shoots. The medium has [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[201],"product_tag":[],"class_list":["post-12428","product","type-product","status-publish","product_brand-duchefa","product_cat-ptc-media","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nRugini Olive Medium - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/rugini-olive-medium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rugini Olive Medium - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"The Olive (Olea europaea sativa L.) plays an important role in the economies of countries in the Mediterranean area. The in vitro culture of Olive, a particular difficult species to propagate in vitro, required the development of a specific medium formulation. Rugini medium is dedicated to the proliferation of the olive shoots. 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