{"id":12421,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12421"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"nln-medium","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/nln-medium\/","title":{"rendered":"NLN medium"},"content":{"rendered":"
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The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg\/l. To prepare the proper NLN medium formulation 500 mg\/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg\/l. To prepare the proper NLN medium formulation 500 mg\/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Composition<\/a><\/h3>\n
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\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CoCl2<\/sub>.6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.025<\/td>\n0.10<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n10.00<\/td>\n161.73<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n18.95<\/td>\n111.94<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.25<\/td>\n1.03<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n10.00<\/td>\n34.78<\/td>\n<\/tr>\n
CoCl2<\/sub>.6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.025<\/td>\n0.10<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n10.00<\/td>\n161.73<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n18.95<\/td>\n111.94<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.25<\/td>\n1.03<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n10.00<\/td>\n34.78<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
Ca(NO3<\/sub>)2.4H2<\/sub>O<\/td>\nOmmitted (500 mg\/l)<\/td>\n<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n125.00<\/td>\n0.92<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n125.00<\/td>\n1.24<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n61.00<\/td>\n0.51<\/td>\n<\/tr>\n
Ca(NO3<\/sub>)2.4H2<\/sub>O<\/td>\nOmmitted (500 mg\/l)<\/td>\n<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n125.00<\/td>\n0.92<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n125.00<\/td>\n1.24<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n61.00<\/td>\n0.51<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Total concentration Micro and Macro elements: 386.95 mg\/l<\/p>\n

Total concentration Micro and Macro elements: 386.95 mg\/l<\/p>\n

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982<\/p>\n

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982<\/p>\n

Hazard statements<\/h4>\n
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  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n
  • H412<\/span>\u00a0Harmful to aquatic life with long lasting effects<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
      \n
    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P273<\/span>\u00a0Avoid release to the environment<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n
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      <\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg\/l. [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[201],"product_tag":[],"class_list":["post-12421","product","type-product","status-publish","product_brand-duchefa","product_cat-ptc-media","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nNLN medium - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/nln-medium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NLN medium - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"The composition of the NLN medium originated from the medium described by Nitsch. 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