{"id":12419,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12419"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"nitsch-medium","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/nitsch-medium\/","title":{"rendered":"Nitsch medium"},"content":{"rendered":"

The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The plantlets matured and flowered profusely, but did not set seed.<\/p>\n

Composition<\/a><\/h3>\n\n\n\n\n\n\n\n\n\n\n
Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.025<\/td>\n<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n10.00<\/td>\n<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n18.94<\/td>\n<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.25<\/td>\n<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n10.00<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
CaCl2<\/sub><\/td>\n166.00<\/td>\n<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n68.00<\/td>\n<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n950.00<\/td>\n<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n90.27<\/td>\n<\/td>\n<\/tr>\n
NH4<\/sub>NO3<\/sub><\/td>\n720.00<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Nitsch J.P. and Nitsch C., Haploid plants from pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis in Nicotiana, Phytomorphology 19, 389 (1969).<\/p>\n

Hazard statements<\/h4>\n
    \n
  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n
  • H412<\/span>\u00a0Harmful to aquatic life with long lasting effects<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
      \n
    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P273<\/span>\u00a0Avoid release to the environment<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

      The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[201],"product_tag":[],"class_list":["post-12419","product","type-product","status-publish","product_brand-duchefa","product_cat-ptc-media","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nNitsch medium - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/nitsch-medium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nitsch medium - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. 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