{"id":12399,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=12399"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"gresshoff-doy-medium","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/gresshoff-doy-medium\/","title":{"rendered":"Gresshoff & Doy medium"},"content":{"rendered":"
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The medium defined by Gresshoff and Doy is developed for growth of haploid callus and plants of Arabidopsis thaliana cultured from the diploid anthers. The anthers were removed during the late prophase of meiosis, selecting a genotype favouring callus formation from dividing sporocytes on a high auxin \u2013 low kinetin concentration in a fully defined medium. Further differentiati-on was induced by transfer to a low auxin \u2013 high kinetin medium with a light-dark cycle. Haploid callus cultures of tomato, barley and Vitis vinifera have been cultured as well using this method.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Composition<\/a><\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n
Micro Elements<\/th>\nmg\/l<\/th>\n\u00b5M<\/th>\n<\/tr>\n<\/thead>\n
CoCl2<\/sub>.6H2<\/sub>O<\/td>\n0.025<\/td>\n0.11<\/td>\n<\/tr>\n
CuSO4<\/sub>.5H2<\/sub>O<\/td>\n0.025<\/td>\n0.10<\/td>\n<\/tr>\n
FeNaEDTA<\/td>\n36.70<\/td>\n100.00<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n0.30<\/td>\n4.85<\/td>\n<\/tr>\n
KI<\/td>\n0.80<\/td>\n4.82<\/td>\n<\/tr>\n
MnSO4<\/sub>.H2<\/sub>O<\/td>\n1.00<\/td>\n5.92<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>.2H2<\/sub>O<\/td>\n0.025<\/td>\n0.10<\/td>\n<\/tr>\n
ZnSO4<\/sub>.7H2<\/sub>O<\/td>\n0.30<\/td>\n1.04<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n
Macro Elements<\/th>\nmg\/l<\/th>\nmM<\/th>\n<\/tr>\n<\/thead>\n
Ca(NO3<\/sub>)2.2H2<\/sub>O<\/td>\n208.81<\/td>\n1.04<\/td>\n<\/tr>\n
KCl<\/td>\n65.00<\/td>\n0.87<\/td>\n<\/tr>\n
KH2<\/sub>PO4<\/sub><\/td>\n300.00<\/td>\n2.20<\/td>\n<\/tr>\n
KNO3<\/sub><\/td>\n1000.00<\/td>\n9.89<\/td>\n<\/tr>\n
MgSO4<\/sub><\/td>\n17.09<\/td>\n0.14<\/td>\n<\/tr>\n
NH4<\/sub>NO3<\/sub><\/td>\n1000.00<\/td>\n12.49<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Gresshoff P.M. et al., Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. Biol. Sci, 25, 259 (1972). Gresshoff P.M. et al. , Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera, Z. Pflanzenphysiol. 73, 132-141, (1974).<\/p>\n

Hazard statements<\/h4>\n
    \n
  • H315<\/span>\u00a0Causes skin irritation<\/li>\n
  • H319<\/span>\u00a0Causes serious eye irritation<\/li>\n
  • H335<\/span>\u00a0May cause respiratory irritation<\/li>\n<\/ul>\n

    Precautionary statements<\/h4>\n
      \n
    • P261<\/span>\u00a0Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray<\/li>\n
    • P302<\/span>\u00a0IF ON SKIN:<\/li>\n
    • P305<\/span>\u00a0IF IN EYES:<\/li>\n
    • P351<\/span>\u00a0Rinse continuously with water for several minutes<\/li>\n
    • P352<\/span>\u00a0Wash with soap and water<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      The medium defined by Gresshoff and Doy is developed for growth of haploid callus and plants of Arabidopsis thaliana cultured from the diploid anthers. The anthers were removed during the late prophase of meiosis, selecting a genotype favouring callus formation from dividing sporocytes on a high auxin \u2013 low kinetin concentration in a fully defined [\u2026]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"inline_featured_image":false},"product_brand":[190],"product_cat":[201],"product_tag":[],"class_list":["post-12399","product","type-product","status-publish","product_brand-duchefa","product_cat-ptc-media","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nGresshoff & Doy medium - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/gresshoff-doy-medium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gresshoff & Doy medium - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"The medium defined by Gresshoff and Doy is developed for growth of haploid callus and plants of Arabidopsis thaliana cultured from the diploid anthers. 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