{"id":10684,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&p=10684"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"mag-bind-endo-free-plasmid-midi-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/mag-bind-endo-free-plasmid-midi-kit\/","title":{"rendered":"Mag-Bind\u00ae Endo-free Plasmid Midi Kit"},"content":{"rendered":"
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Ready-to-implement automated purification solution<\/h3>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
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All Mag-Bind\u00ae kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors.\u00a0Learn more.<\/a><\/strong><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Off-the-shelf product doesn\u2019t suit your needs? We\u2019ll work with you to develop a customized product.\u00a0Learn more.<\/a><\/strong><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications.\u00a0Learn more.<\/strong><\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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The Mag-Bind\u00ae Endo-free Plasmid Midi Kit is designed to deliver large scale purification of high-quality plasmid DNA with endotoxins levels <0.1 EU\/\u00b5g for use in eukaryotic transfections and other sensitive in vitro applications.<\/p>\n

The Kit follows magnetic bead-based technology to purify plasmid DNA from up to 50 mL bacterial culture in LB or up to 10 mL bacterial culture in TB or other suitable growth medium. The Mag-Bind\u00ae Endo-free Plasmid DNA Kit can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Yields may vary depending on the copy number of the plasmid, E. coli strain used, culture media, and condition of growth.<\/p>\n

The Mag-Bind\u00ae Endo-free Plasmid Midi Kit follows a modified alkaline lysis method forming a bacterial lysate. The lysate is then cleared using either Mag-Bind\u00ae Particles LC (sold separately) or Lysate Clearance Filter Syringe (sold separately) or centrifugation. The novel paramagnetic lysate clearance beads bind to cell debris and pull them down when magnetized. This eliminates the need for centrifugation or vacuum manifold for lysate clearance, making this suitable for high-throughput, automation workflows.<\/p>\n

Post lysate clearance, plasmid DNA is then bound to Mag-Bind\u00ae Particles RQ through a specially formulated binding buffer and the Mag-Bind\u00ae particles are subjected to a wash routine to remove endotoxins, salt, and other contaminants. High-quality plasmid DNA is then eluted in Endo-free Water and is ready for use in a wide range of downstream applications such as transfection routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing, etc.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Purify endotoxin free (<0.1 EU\/\u00b5g) plasmid DNA using magnetic beads from up to 50 mL culture volume.<\/em><\/p>\n

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  • Fully Automatable<\/b>\u00a0\u2013 Magnetic beads clear the lysate, avoiding the manual centrifugation step<\/li>\n
  • High-yielding<\/strong>\u00a0\u2013 Plasmid DNA yield up to 200 \u03bcg<\/li>\n
  • Rapid<\/strong>\u00a0\u2013 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention<\/li>\n
  • Versatile<\/b>\u00a0\u2013 Process up to 50 mL bacterial culture with different plasmid types and culture medias<\/li>\n
  • Transfection-quality<\/b>\u00a0\u2013 Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU\/\u00b5g<\/li>\n
  • Pure<\/b>\u00a0\u2013 Contamination-free plasmid DNA with A260\/A280 > 1.8 and A260\/A230 > 1.8<\/li>\n<\/ul>\n

    M1272-00S Mag-Bind\u00ae Endo-free Plasmid Midi Kit (Sample Request)<\/p>\n","protected":false},"featured_media":10685,"template":"","meta":{"inline_featured_image":false},"product_brand":[195],"product_cat":[117],"product_tag":[],"class_list":{"0":"post-10684","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-omega","7":"product_cat-plasmid-dna","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nMag-Bind\u00ae Endo-free Plasmid Midi Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/mag-bind-endo-free-plasmid-midi-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Mag-Bind\u00ae Endo-free Plasmid Midi Kit - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Purify endotoxin free (<0.1 EU\/\u00b5g) plasmid DNA using magnetic beads from up to 50 mL culture volume. 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