{"id":10669,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&#038;p=10669"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"e-z-n-a-fastfilter-plasmid-dna-midi-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/e-z-n-a-fastfilter-plasmid-dna-midi-kit\/","title":{"rendered":"E.Z.N.A.\u00ae FastFilter Plasmid DNA Midi Kit"},"content":{"rendered":"<p>The E.Z.N.A.\u00ae FastFilter Plasmid DNA Midi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple \u201cbind-wash-elute\u201d procedure to deliver high quality plasmid DNA. The yields vary according to plasmid copy number,\u00a0<em>E. coli<\/em>\u00a0strain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 \u00b5g of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR,\u00a0<em>in vitro<\/em>\u00a0transcription, transformation, and other applications.<\/p>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>Speed \u2013 Purification of plasmid DNA &lt; 40 minutes<\/li>\n<li>Safety \u2013 No phenol\/chloroform extractions<\/li>\n<li>Versatile \u2013 Spin and vacuum formats available<\/li>\n<li>High quality \u2013 DNA is suitable for a variety of downstream applications<\/li>\n<\/ul>\n<p>D6905-00S E.Z.N.A.\u00ae FastFilter Plasmid DNA Midi Kit (Sample Request)<\/p>\n","protected":false},"featured_media":10670,"template":"","meta":{"inline_featured_image":false},"product_brand":[195],"product_cat":[117],"product_tag":[],"class_list":{"0":"post-10669","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-omega","7":"product_cat-plasmid-dna","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>E.Z.N.A.\u00ae FastFilter Plasmid DNA Midi Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/e-z-n-a-fastfilter-plasmid-dna-midi-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"E.Z.N.A.\u00ae FastFilter Plasmid DNA Midi Kit - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Speed \u2013 Purification of plasmid DNA &lt; 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