{"id":10667,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&#038;p=10667"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"e-z-n-a-fastfilter-plasmid-dna-maxi-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/e-z-n-a-fastfilter-plasmid-dna-maxi-kit\/","title":{"rendered":"E.Z.N.A.\u00ae FastFilter Plasmid DNA Maxi Kit"},"content":{"rendered":"<p>The E.Z.N.A.\u00ae FastFilter Plasmid DNA Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple \u201cbind-wash-elute\u201d procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number,\u00a0<em>E. coli<\/em>\u00a0strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR,\u00a0<em>in vitro<\/em>\u00a0transcription, transformation, and other applications.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u2022 Cost and time savings compared to anion exchange technology<br \/>\n\u2022 Spin and vacuum formats available<br \/>\n\u2022 Lysate Clearance Syringes to rapidly clear bacterial lysates post-lysis<\/p>\n<p>D6924-00S E.Z.N.A.\u00ae FastFilter Plasmid DNA Maxi Kit (Sample Request)<\/p>\n","protected":false},"featured_media":10668,"template":"","meta":{"inline_featured_image":false},"product_brand":[195],"product_cat":[117],"product_tag":[],"class_list":{"0":"post-10667","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-omega","7":"product_cat-plasmid-dna","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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